化學(xué)發(fā)光標記筆介紹

WesternBright 化學(xué)發(fā)光標記筆，可直接在預(yù)染的蛋白質(zhì)marker上或者蛋白轉(zhuǎn)移膜上書寫，其*的“墨水”可以和HRP底物（ECL發(fā)光液）起反應(yīng)，產(chǎn)生化學(xué)發(fā)光信號。
優(yōu)勢： 
更簡便-- 可將蛋白質(zhì)Marker轉(zhuǎn)換成化學(xué)發(fā)光條帶，和目的蛋白質(zhì)條帶一起同時成像，方便觀察和分析
更人性化-- 可直接在蛋白轉(zhuǎn)移膜上注明編號和日期，方便實驗數(shù)據(jù)的管理
確認 HRP 底物穩(wěn)定性-- ChemiPen中的試劑和HRP底物（ECL發(fā)光液）結(jié)合后可長時間發(fā)光，所以他可以用來實時檢測HRP底物（ECL發(fā)光液）的穩(wěn)定性
兼容性-- 可用于膠片和CCD成像

技術(shù)參數(shù)

You can use the WesternBright ChemiPen to transform your visible protein markers into chemiluminescent markers, to annotate your blot with a date or blot ID, or to check the stability of your HRP substrate (Figure 1).

ChemiPen Figure 1 

Figure 1. The WesternBright ChemiPen can be used to mark the location of protein standards and to annotate the blot with a date, as in the right panel.

Marking the location of visible protein standards on a blot is easy. Simply trace the visible markers on the membrane after transfer and before blocking (Figure 2).

ChemiPen Figure 2 

Figure 2. Use the WesternBright ChemiPen to visualize visible protein standards on chemiluminescent Western blots

The WesternBright ChemiPen has two tips, a fine tip at one end that is perfect for writing on the blot or tracing the bands of a visible protein standard, and a thicker wedge tip that can be used to deposit greater amounts of reagent on the blot.